ELECTRON MICROSCOPE EXAMINATION OF SUBCELLULAR FRACTIONS
نویسندگان
چکیده
منابع مشابه
Electron Microscope Examination of Subcellular Fractions
Rat liver microsomes and microsomal subfractions isolated by density equilibration were submitted to a quantitative morphological and biochemical analysis. The total area of the endoplasmic reticulum was estimated at 7.3 m(2) per g of liver. The microsome fraction contained 2.8 mg of phospholipids and 6.7 mg of proteins per m(2) of membrane area. After correction for ribosomal and intracisterna...
متن کاملElectron Microscopic Examination of Subcellular Fractions
Large granule fractions, containing about 80% of the cytochrome oxidase of the tissue, were isolated from rat liver and used to prepare thin pellicles of packed particles which were submitted to quantitative electron microscopic examination. Various parameters describing the mitochondrial population were determined by measuring the size and number of mitochondrial profiles in sections, and the ...
متن کاملAn electron-microscope study of subcellular fractions of Octopus brain.
Using subcellular fractionation techniques the primary fractions (P1; P2 and P3) have been prepared from homogenates of the supraoesophageal lobes (brain) of Octopus vulgaris. A morphological study of the fractions was made, particular emphasis being placed upon the synaptosomes (nerve-ending particles), and the agranular and granular vesicles they contain. The acetylcholine levels in the fract...
متن کاملELECTRON MICROSCOPE EXAMINATION OF SUBCELLULAR FRACTIONS III. Quantitative Analysis of the Microsomal Fraction Isolated from Rat Liver
Morphometric studies of the cellular membranes have been carried out on normal rat liver by Loud (29) and by Weibel et al . (47) . Such measurements in situ offer the advantage of allowing easy cytological identification of the structures, but they do not permit direct correlation between morphological and biochemical data on the same material . Such a correlation can be more directly establish...
متن کاملELECTRON MICROSCOPIC EXAMINATION OF SUBCELLULAR FRACTIONS II. Quantitative Analysis of the Mitochondrial Population Isolated from Rat Liver
Large granule fractions, containing about 80 % of the cytochrome oxidase of the tissue, were isolated from rat liver and used to prepare thin pellicles of packed particles which were submitted to quantitative electron microscopic examination. Various parameters describing the mitochondrial population were determined by measuring the size and number of mitochondrial profiles in sections, and the...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1971
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.51.1.52